Studies on improved Agrobacterium-mediated transformation in two indica rice (Oryza sativa L.)
نویسندگان
چکیده
Agrobacterium tumefaciens strain EHA 105 carrying binary vector pCAMBIA 1301 was used for transformation in two economically important highly recalcitrant indica rice cultivars HKR-46 and HKR126. High concentrations of acetosyringone in the Agrobacterium culture and co-cultivation medium proved to be indispensable for successful transformation. Embryogenic scutellar calli were used for transformation studies. Binary vector pCAMBIA 1301 have been proved efficient for transformation. The percent transient GUS expression found to be higher in cutivar HKR-126 (44.4%) as compared to HKR46 (28.9%). The percent recovery of hygromycin resistant calli after 4-6 weeks on selection medium was maximum in HKR-126 (52.6%).
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